By Johanna Maria Ticar
The grasp thesis of Johanna Maria Ticar finds high-resolution insights into the myocardial microstructure and illustrates that cardiac muscle fibers are immediately, operating in parallel with one most well-liked fiber course, besides the fact that, deposits comparable to fats appear to compromise the typical and compact constitution. moment harmonic new release imaging mixed with optical tissue clearing is a correct technique for deciding on the 3-dimensional muscle fiber and sheet orientations and accordingly, permits the calculation of fiber rotation through the ventricle wall.
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Extra resources for 3D Analysis of the Myocardial Microstructure: Determination of Fiber and Sheet Orientations
In both (a) and (b) the organization of ﬁbers in sheets is visible. 6: Volume rendering of optical sections obtained by SHG imaging. Previous to the actual imaging the sample was rotated 90◦ around the x-axis. (a) is an in-plane (xz) projection of the top of the volume-rendered sample. 7 depicts two different samples, both having a depth of 500 μm with a step size of 5 μm in between individual slices and in-plane dimension 620×620 μm. 14) is higher. 1 as this sample was considered to be an outlier.
10 depicts the three-dimensional myocytes ﬁber orientations of a z-stack with a depth of approximately 1060 μm (y-axis). The horizontal lines correspond to about 54 images of which the distribution of ﬁber angles and amplitudes were indivdually determined. 11: Depiction of the von Mises distribution ρ(Φ) for ﬁve different concentration parameters b. ρ(Φ)=1 is true for b=0 characterized by a uniform distribution representing an isotropic ﬁber distribution. A Dirac delta function at angle μ represents the distribution for b −→ ∞ (Schrieﬂ et al.
2). From the ﬁt, both the location parameter μ and the concentration parameter b were obtained and calculated for every single image in the z-stack. The location parameter μ describes the principal (preferred) ﬁber orientation of the distribution, while the concentration parameter b determines the shape of the von Mises distribution. A narrower distribution indicates elevated b-values and hence, more aligned ﬁbers. Therefore, a more anisotropic ﬁber distribution follows. Both structural parameters are plotted over the depth, shown in (a) and (b) of Fig.
3D Analysis of the Myocardial Microstructure: Determination of Fiber and Sheet Orientations by Johanna Maria Ticar